Key result
Optimized guidewire-induced aortic valve injury protocol in mice achieves ~90% model success at four weeks.
Why the study?
Aortic valve stenosis prevalence is increasing, yet the scarcity of diseased valve tissue limits mechanistic studies and therapeutic development.
An optimized guidewire-induced aortic valve injury protocol using a 3D-printed support platform achieves high survival and reproducibility in mice, providing a reliable model for AVS research.
Answers are grounded in this paper and cite their sources.
Optimizes reproducible mouse AVS model; leaves open translation to human therapies pending validation.
Aortic valve stenosis (AVS) is a major cardiovascular disease with increasing prevalence in aging populations, yet the scarcity of diseased valve tissue severely limits mechanistic studies and therapeutic development. This protocol establishes and optimizes a reproducible mouse model of AVS by improving upon the Honda guidewire-induced valve injury method through controlled hemorrhage management. Male and female C57BL/6 mice undergo guidewire-induced aortic valve injury; the procedure is refined by a custom 3D-printed vascular support platform that achieves precise hemorrhage control during guidewire insertion and withdrawal. Outcome measures include 24 h survival, echocardiographic assessment at 4 weeks, and histopathological validation. The optimized protocol achieves a 95% 24 h survival rate (19 of 20 animals); among survivors, 94.7% (18 of 19) meet the predefined hemodynamic success criterion at 4 weeks, yielding an overall model success rate of 90% (18 of 20 animals), confirmed by echocardiography and histological analysis. This standardized model provides a reliable, reproducible platform for investigating mechanisms of valve degeneration and evaluating candidate therapeutic interventions.
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Zhang et al. (2026) studied Aortic valve stenosis (n=20). Optimized guidewire-induced aortic valve injury protocol was evaluated on Overall model success rate (24 h survival and hemodynamic success at 4 weeks). An optimized guidewire-induced aortic valve injury protocol in mice achieved a 95% 24-hour survival rate and an overall model success rate of 90% at 4 weeks.
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