Key result
Overexpression of IGFBP2 in primary cultured chicken hepatocytes significantly increased the expression of PPARG, IGF1, and IGF1R genes as well as triglyceride production.
Why the study?
The effects of insulin-like growth factor binding protein 2 on the expression of fatty acid synthesis regulators and triglyceride production in primary cultured chicken hepatocytes were investigated.
p-value: p=<0.05
IGFBP2 overexpression in chicken hepatocytes increases the expression of fatty acid synthesis regulators and triglyceride production, suggesting a role in lipogenesis.
IGFBP2 overexpression may drive hepatic triglyceride accumulation in chickens; hypothesis-generating for avian lipid metabolism pending in vivo confirmation.
The effects of insulin-like growth factor binding protein 2 (IGFBP2) on the expression of fatty acid synthesis regulators and triglyceride production were investigated in primary cultured chicken hepatocytes. The full-length chicken IGFBP2 coding region was synthesized by overlap extension PCR and cloned into the pcDNA3.1 vector. An in situ digestion method was used to prepare the chicken hepatocytes. Primary chicken hepatocytes were maintained in monolayer culture. Real-time PCR was used to detect changes in the expression of IGFBP2, PPARG, IGF1, IGF1R, APOAI, and LFABP, after the overexpression of IGFBP2 in chicken hepatocytes. Triglyceride production and glucose content were also evaluated using triglyceride and glucose analysis methods. The expression level of IGFBP2 increased after transfection of the IGFBP2-containing vector. The expression levels of PPARG, IGF1, and IGF1R also increased in cultured chicken hepatocytes after the overexpression of IGFBP2, whereas the expression of LFABP and APOAI decreased. Triglyceride production in primary cultured chicken hepatocytes increased after the overexpression of IGFBP2. These results suggest that IGFBP2 is involved in lipogenesis, increasing both the expression of fatty acid synthesis regulators, and triglyceride production in primary cultured chicken hepatocytes.
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Mu et al. (2019) studied Lipogenesis in chicken hepatocytes (n=3). IGFBP2 overexpression vs. pcDNA3.1 vector and untransfected cells was evaluated on Triglyceride production and expression of fatty acid synthesis regulators (p=<0.05). Overexpression of IGFBP2 in primary cultured chicken hepatocytes significantly increased the expression of PPARG, IGF1, and IGF1R genes as well as triglyceride production.
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