What question did this study set out to answer?

Design and evaluate a low-cost, high-throughput flow cytometer using a consumer webcam and wide-field illumination to detect rare target cells in large fluid volumes.

January 1, 2014The Analyst

Webcam-based flow cytometer using wide-field imaging for low cell number detection at high throughput

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Authors

JBJoshua BalsamCenter for Devices and Radiological Health HBHugh A. BruckUniversity of Maryland, College Park ARAvraham RasoolyCenter for Food Safety and Applied Nutrition

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Implication

Experimental study demonstrates high-throughput detection of rare cells in blood using a webcam-based cytometer, highlighting potential for low-cost point-of-care diagnostics.

Key Points

Design and evaluate a low-cost, high-throughput flow cytometer using a consumer webcam and wide-field illumination to detect rare target cells in large fluid volumes.
Constructed a system combining a 187 fps commercial webcam (320 × 240 pixel resolution), a 1 W 450 nm laser module, and a custom 2D flow-cell designed to eliminate motion blur.
Tested detection efficiency and throughput using fluorescent 5 μm polystyrene beads and SYTO-9-stained THP-1 human monocytes suspended in buffer and whole blood at 500 μL min⁻¹.
The platform successfully detected fluorescent target cells at concentrations down to 1 cell per mL at 500 μL min⁻¹ in both buffer and blood suspensions.
Detection sensitivity compared to standard microscopy was concentration-dependent: 84% at 100 cells per mL, 79% at 10 cells per mL, and 59% at 1 cell per mL.
Whole blood samples spiked with 100 cells per mL yielded an average detected concentration of 91.4 cells per mL (95% CI, 86–97 cells per mL).

Cite This Study

Balsam et al. (2014) studied this question.

synapsesocial.com/papers/6a8af15952e1a91077e951a9 https://doi.org/10.1039/c4an00669k

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Implication

Experimental study demonstrates high-throughput detection of rare cells in blood using a webcam-based cytometer, highlighting potential for low-cost point-of-care diagnostics.

Webcam-based flow cytometer using wide-field imaging for low cell number detection at high throughput

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Implication

Key Points

Cite This Study

Webcam-based flow cytometer using wide-field imaging for low cell number detection at high throughput

Discussion

Member takes

Implication

Key Points

Cite This Study

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