The DA strain of Theiler's murine encephalomyelitis virus can serve as a vector for the introduction and expression of foreign genes, such as human lymphotoxin.
Supports viral vector use for foreign gene expression in cell culture; leaves open in vivo translation and therapeutic applications.
Theiler's murine encephalomyelitis virus (TMEV) belongs to the Picornaviridae genus. DA subgroup strains of this virus induce early, non-fatal polioencephalomyelitis followed by demyelination in the spinal cord, with virus persistence. We investigated the use of DA strain as a vector for the introduction of a foreign gene into the central nervous system. Human lymphotoxin (LT) gene was inserted in the L region, the most upstream part of the polyprotein coding region of DA genome. Expression of LT was demonstrated by an immunoblot and an enzyme-linked immunosorbent assay on BHK-21 cells that were infected with the recombinant virus. In addition, the expressed LT showed cytotoxicity against L-929 cells.
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Obuchi et al. (1999) studied this question.
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