What question did this study set out to answer?

To determine whether porcine brain natriuretic peptide regulates steroidogenesis and cyclic GMP production in bovine adrenocortical cells and to characterize its specific receptor binding sites.

August 29, 1988FEBS Letters

Porcine brain natriuretic peptide, another modulator of bovine adrenocortical steroidogenesis

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To determine whether porcine brain natriuretic peptide regulates steroidogenesis and cyclic GMP production in bovine adrenocortical cells and to characterize its specific receptor binding sites.
Evaluated aldosterone and cortisol secretion alongside cyclic GMP accumulation in dispersed bovine adrenocortical cells stimulated with an angiotensin II analog and ACTH.
Characterized binding sites in bovine adrenal cortex using in vitro receptor autoradiography and 125I-labeled porcine brain natriuretic peptide competitive binding assays on isolated membranes.
Porcine brain natriuretic peptide significantly inhibited stimulated aldosterone and cortisol production while simultaneously increasing cyclic GMP formation in adrenocortical cells.
Membrane binding assays identified high-affinity, low-capacity binding sites with a dissociation constant (Kd) of 2.32 ± 0.33 × 10⁻¹⁰ M and a maximal binding capacity (Bmax) of 36.7 ± 1.6 fmol/mg protein.
Specific radioligand binding was completely displaced by both unlabeled porcine brain natriuretic peptide (IC50 = 6.5 × 10⁻¹⁰ M) and human atrial natriuretic peptide (IC50 = 8.5 × 10⁻¹⁰ M).

Structured PICO

Population

Dispersed bovine adrenocortical cells and bovine adrenal gland/cortex membrane fractions

Intervention

Porcine brain natriuretic peptide (pBNP)

Outcome

Aldosterone production, cortisol secretion, cGMP formation, and receptor binding characteristics (Kd, Bmax, IC50)surrogate

pBNP exerts a suppressive effect on bovine adrenocortical steroidogenesis via a receptor shared with ANP.

Discussion

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Implication

May inhibit adrenocortical steroidogenesis via shared ANP receptors in vitro; hypothesis-generating for BNP effects in vivo.

Cite This Study

Hashiguchi et al. (1988) studied this question.

synapsesocial.com/papers/6a8afbe4c716e89f35deccd6 https://doi.org/10.1016/0014-5793(88)80076-0

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Porcine brain natriuretic peptide, another modulator of bovine adrenocortical steroidogenesis

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Porcine brain natriuretic peptide, another modulator of bovine adrenocortical steroidogenesis

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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