Since Feulgen introduced his “Nuclealreaktion” for the demonstration of nucleoproteids, several papers have been published by Continental workers recording the results of the application of this method to a wide range of animal cells. The general consensus of opinion is that the method provides a precise microchemical colour reaction for thymus-nucleic acid protein complexes. No other cell structures, or inclusions of any kind are stained, when the correct procedure is employed.
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Reginald James Ludford (1928) studied this question.
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