Evaluation of cellulose extraction procedures for preparing tree ring samples for carbon isotopic analysis reveals that cellulose can be best extracted with a C6H6-C2H5OH mixture in a Soxhlet apparatus and then soaked and bleached in NaClO2 + CH3COOH solutions. Moreover, systematic measurements of δ13C of tree rings show that they vary considerably across rings and also vary around rings in different radial directions, a fact of great importance in interpretation of tree ring δ13C time series records for past climatic conditions.
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Sheu et al. (1995) studied this question.
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