Key Points
- To identify and characterize the activation-specific platelet surface receptor PADGEM (P-selectin) and delineate its intracellular translocation upon stimulation.
- Generated and evaluated monoclonal antibodies (KC4 and S12) specific to antigens exposed exclusively on activated platelets.
- Conducted immunofluorescence staining on resting, permeabilized, and thrombin-stimulated platelets to track receptor localization.
- Identified that PADGEM is identical to granule membrane protein 140 (GMP-140), LECAM-3, and CD62, which is now designated as P-selectin.
- Demonstrated internal punctate fluorescence in permeabilized resting platelets and rim surface staining in intact thrombin-activated platelets, confirming the translocation of PADGEM from intracellular storage granules to the plasma membrane.
Structured PICO
PPopulationPlatelets (in vitro)
OOutcomeIdentification and localization of PADGEM (P-selectin) on activated platelets
Describes the identification and mechanism of PADGEM (P-selectin) as an adhesion receptor expressed on the surface of platelets following activation.