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January 1, 1982Cold Spring Harbor Symposia on Quantitative Biology

Regulation of Myosin-filament Assembly by Light-chain Phosphorylation

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Authors

JKJohn Kendrick‐JonesHeart Failure / CardiomyopathyPTP. ToothMRC Laboratory of Molecular BiologyKTKenneth A. TaylorFlorida State University

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Implication

Stable myosin filaments remain intact in striated muscle; leaves open dynamic organization in nonmuscle cardiovascular cells.

Key Points

  • Determine the molecular organization of nonmuscle myosin and understand how light-chain phosphorylation regulates the assembly of myosin filaments relative to actin.
  • In vitro biochemical and ultrastructural analysis of vertebrate nonmuscle myosin assembly into bipolar filaments.
  • Comparison of striated-muscle and nonmuscle myosin stability, concentration, and structural organization under varying phosphorylation states.
  • Vertebrate nonmuscle myosins assemble into short bipolar filaments in vitro, differing from the stable, persistent filament structures seen in striated muscle.
  • Nonmuscle myosin filaments are infrequently observed by electron microscopy in intact cells, likely owing to low intracellular myosin concentrations and small filament dimensions.

Cite This Study

Kendrick‐Jones et al. (1982) studied this question.

synapsesocial.com/papers/6a8b9922df3a83baf8caa69chttps://doi.org/10.1101/sqb.1982.046.01.087
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Assembly of smooth muscle myosin into side-polar filaments.1977 · 155 citations
  2. 2Structure and Function of Chicken Gizzard Myosin11978 · 268 citations
  3. 3An electrophoretic study of the low-molecular-weight components of myosin1970 · 711 citations
  4. 4Electron microscopic localization of cytoplasmic myosin with ferritin-labeled antibodies.1981 · 77 citations
  5. 5Localization of myosin, actin, and tropomyosin in rat intestinal epithelium: immunohistochemical studies at the light and electron microscope levels.1980 · 138 citations