Eighteen sexual clones of Closterium belonging to the C. venus - C. dianae complex were isolated and studied in unialgal culture. No correlation could be found between vegetative structure and the type of sexual morphology. The features of sexual reproduction appear to be less variable within a strain and to show less intergradation between different strains than the various aspects of the vegetative cells. It is proposed that sexual morphology be an essential feature in the identification and description of taxa within this group of Closterium. Closterium evesiculatum is described as a new species.
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Philip W. Cook (1963) studied this question.