SUMMARY The distribution of ice crystal sizes in frozen thin sections correlates with the size distribution in freeze fracture replicas. In the hypothetical case of melting (and refreezing) during cyro‐sectioning this correlation should be lost as was found after intentional melting or frozen thin sections. These observations demonstrate that there is neither a through section melting nor a growth of ice crystals during cryo‐ultramicrotomy.
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Frederik et al. (1981) studied this question.
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