Why the study?
Despite its essential role in striated muscle regulation, the structure of troponin has remained elusive due to its high structural heterogeneity.
Provides a structural basis for understanding the molecular mechanism of the actomyosin system in cardiac muscle.
Cardiac thin filament structures may guide troponin research in contractile disorders; leaves open clinical translation.
Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.
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Oda et al. (2020) studied this question.
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