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In the University of California Botanical Garden over 60 species and varieties of the genus Nicotiana have been under investigation, together with species of such related genera as Petunia, Fabiana, Salpiglossis and Nierembergia.Morphological, distributional and cytogenetic evidence indicates the desirability of recognizing among the races of Nicotiana under observation, the following 40 species.The list is arranged in order of date of description.
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T. H. Goodspeed (1933) studied this question.
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