What question did this study set out to answer?

To determine whether endogenous guanylin regulates transepithelial chloride secretion in human colonic T84 cells through cyclic guanosine monophosphate signaling, mirroring the action of bacterial enterotoxins.

June 1, 1993Journal of Clinical InvestigationOpen Access

Guanylin stimulation of Cl- secretion in human intestinal T84 cells via cyclic guanosine monophosphate.

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To determine whether endogenous guanylin regulates transepithelial chloride secretion in human colonic T84 cells through cyclic guanosine monophosphate signaling, mirroring the action of bacterial enterotoxins.
Cultured human T84 colonic epithelial cells on semipermeable supports and evaluated chloride secretion by monitoring short-circuit current following apical or basolateral addition of synthetic guanylin or Escherichia coli heat-stable enterotoxin (ST 5-17).
Assessed transepithelial transport sensitivity using basolateral bumetanide and measured intracellular cyclic guanosine monophosphate (cGMP) accumulation.
Conducted competitive radioligand binding assays with 125I-labeled ST on intact T84 monolayers to model receptor affinity parameters (Ki values).
Apical application of guanylin stimulated chloride secretion and induced a >1,000-fold increase in intracellular cGMP, demonstrating approximately one-tenth the secretory potency of E. coli ST 5-17; both secretory responses were inhibited by basolateral bumetanide.
Radioligand competition assays fit a two-receptor model yielding Ki values of 19 ± 5 nM and 1.3 ± 0.5 µM for guanylin, compared to 78 ± 38 pM and 4.9 ± 1.4 nM for ST 5-17.

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Implication

In vitro study demonstrates guanylin stimulation of chloride secretion via cGMP in human intestinal T84 cells, indicating bacterial enterotoxins mimic an endogenous fluid regulation pathway.

Cite This Study

Forte et al. (1993) studied this question.

synapsesocial.com/papers/6a8bcceaeffeb02058b1eb96 https://doi.org/10.1172/jci116476

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Implication

In vitro study demonstrates guanylin stimulation of chloride secretion via cGMP in human intestinal T84 cells, indicating bacterial enterotoxins mimic an endogenous fluid regulation pathway.

Guanylin stimulation of Cl- secretion in human intestinal T84 cells via cyclic guanosine monophosphate.

Key Points

Discussion

Implication

Cite This Study

Guanylin stimulation of Cl- secretion in human intestinal T84 cells via cyclic guanosine monophosphate.

Key Points

Discussion

Implication

Cite This Study

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