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The new star of Westerns: A highly sensitive fluorescence probe for alkaline phosphatase (ALP) based on a fluorescein derivative has been prepared and found to be suitable for use in Western blot analysis. The probe 1 is nonfluorescent but undergoes hydrolysis in the presence of ALP to yield a green-fluorescent derivative 2 and may be used in multicolor labeling in combination with other probes.
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Kamiya et al. (2005) studied this question.
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