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March 1, 1978AJP Regulatory Integrative and Comparative Physiology

Angiotensin-converting enzyme in developing lung and kidney

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Population

Fetal, newborn, and developing rats and mice (lung and kidney tissues)

Comparison

Observation of age-related development vs Different developmental stages

Design

Preclinical

Authors

KWKendall B. WallaceUniversity of North TexasMBM. D. BailiePfizer (United States)JHJerry B. HookDartmouth College

Discussion

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Implication

Should not yet inform neonatal therapy; extends age-dependent ACE maturation across rodent lungs and kidneys.

Key Points

  • To evaluate developmental changes in angiotensin-converting enzyme (ACE) activity in pulmonary and renal tissues from late gestation through maturation.
  • Assayed tissue homogenates from rat and mouse lung and kidney across developmental stages ranging from near-term fetal animals to 6 weeks postpartum.
  • Quantified ACE activity by measuring the optical density of hippuric acid liberated from hippuryl-L-histidyl-L-leucine following incubation with 20,000 × g tissue supernatants.
  • Assessed substrate affinity to determine whether developmental changes in activity reflected kinetic alterations or enzyme abundance.
  • Pulmonary ACE activity did not differ between near-term fetal and 1-day-old rats, but increased in a biphasic manner over the first 6 weeks postpartum.
  • Rat kidney, mouse kidney, and mouse lung demonstrated a similar age-dependent increase in converting enzyme activity across development.
  • Substrate affinity remained equivalent across all developmental time points, indicating that increased activity was driven by elevated enzyme quantity rather than altered affinity.

Structured PICO

P
Population
Fetal, newborn, and developing rats and mice (lung and kidney tissues)
I
Intervention
Observation of age-related development
C
Comparator
Different developmental stages (near-term fetal, 1-day-old, up to 6 weeks postpartum)
O
Outcome
Angiotensin-converting enzyme (ACE) activitysurrogate

ACE activity increases in an age-dependent manner in developing rat and mouse lungs and kidneys, which may limit angiotensin II production in newborns.

Cite This Study

Wallace et al. (1978) studied this question.

synapsesocial.com/papers/6a8bd60ea42d46e0ffcad05fhttps://doi.org/10.1152/ajpregu.1978.234.3.r141
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1DEVELOPMENT OF ANGIOTENSIN CONVERTING ENZYME IN FETAL LUNG AND PLACENTA OF THE RAT AND HUMAN1984 · 23 citations
  2. 2Fetal, neonatal cord, and maternal plasma concentrations of angiotensin‐converting enzyme (ACE)2002 · 18 citations
  3. 3Ontogeny of somatic angiotensin-converting enzyme.1994 · 78 citations
  4. 4Vascular Angiotensin Converting Enzyme in the Development of Renal Hypertension1986 · 26 citations
  5. 5Angiotensin I–Converting Enzyme Isoforms (High and Low Molecular Weight) in Urine of Premature and Full-Term Infants2000 · 21 citations