An automated method is described for determining alkaline phosphatase activity in serum, with use of sodium thymolphthalein monophosphate as the substrate. The system makes use of standard AutoAnalyzer components, has a simple flow diagram, and does not involve dialysis. The method has good precision, and the results correlate well with those from the manual method of which it is an adaptation
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Proksch et al. (1973) studied this question.
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