Liver Glucokinase Induction in Acute and Chronic Insulin Insufficiency in Rats

Administration of anti-insulin guinea pig serum to normal rats fed with a high fat carbohydrate-free diet prevented almost completely the increase in liver glucokinase activity that is normally induced by the administration of glucose. The simultaneous administration of carbohydrate and insulin to diabetic rats fed for several days with a carbohydrate-free diet induced a rapid increase in glucokinase activity that could be similar to that observed in normal animals fed the same diet. This liver response depended on the dose of insulin in relation to the severity of the diabetic condition.