Antisera which react with methadone and with urine from users of this drug have been prepared in rabbits. A simple and rapid hemagglutination-inhibition test which detects methadone in concentrations of 1 ng/ml has been applied to human urine specimens with results that demonstrate the specificity of the test and suggest that use of the drug may remain detectable for at least 1 week. Heroin used simultaneously with methadone does not interfere in the test.
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Liu et al. (1973) studied this question.