What question did this study set out to answer?

To elucidate the functional contributions of specific glutamate residues (positions 162, 164, and 167) within the omega-loop to SHV-14 beta-lactamase activity and drug resistance.

November 29, 2017FEMS Microbiology Letters

Glutamate residues at positions 162 and 164 influence the beta-lactamase activity of SHV-14 obtained from Klebsiella pneumoniae

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Authors

GKGaurav KumarSRM Institute of Science and Technology SBSarmistha BiswalInstitute of Life Sciences SNSoshina NathanFatima Mata National College

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Implication

In vitro mutational analysis reveals that omega-loop glutamate substitutions reduce beta-lactamase activity in Klebsiella pneumoniae, highlighting new targets for antibiotic inhibitor design.

Key Points

To elucidate the functional contributions of specific glutamate residues (positions 162, 164, and 167) within the omega-loop to SHV-14 beta-lactamase activity and drug resistance.
Substituted individual omega-loop glutamates with alanine in the SHV-14 beta-lactamase gene.
Evaluated beta-lactam susceptibility profiles in transformed Escherichia coli surrogate cells and performed complementary in silico analyses.
Purified wild-type and mutant SHV-14 enzymes to measure catalytic efficiencies for beta-lactam antibiotic hydrolysis.
Wild-type SHV-14 expression increased host resistance against penicillin and cephalosporin antibiotics by 60- to 500-fold.
Alanine mutations at glutamate positions, particularly E164A, substantially reduced antibiotic resistance levels relative to the wild-type enzyme.
Decreases in bacterial drug resistance correlated with marked reductions in catalytic hydrolysis efficiency among the purified mutant proteins.

Cite This Study

Kumar et al. (2017) studied this question.

synapsesocial.com/papers/6a8bed07f07ad6ed43b23afe https://doi.org/10.1093/femsle/fnx259

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Implication

In vitro mutational analysis reveals that omega-loop glutamate substitutions reduce beta-lactamase activity in Klebsiella pneumoniae, highlighting new targets for antibiotic inhibitor design.

Glutamate residues at positions 162 and 164 influence the beta-lactamase activity of SHV-14 obtained from Klebsiella pneumoniae

Discussion

Member takes

Implication

Key Points

Cite This Study

Glutamate residues at positions 162 and 164 influence the beta-lactamase activity of SHV-14 obtained from Klebsiella pneumoniae

Discussion

Member takes

Implication

Key Points

Cite This Study

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