Model of the formation of streptozotocin-induced diabetes in Wiesenau pigs with assessment of its effectiveness and stability

BACKGROUND: Research aimed at creating and validating models of diabetes plays an important role in the development of new treatments for this disease. Large animal models may bridge the gap between basic research and clinical trials of new technologies for the treatment of insulin deficiency. At the moment, the most popular model is streptozotocin-induced diabetes. However, different species and breeds of animals respond differently to streptozotocin. Thus, although models already exist in different breeds of pigs, verification of the Wiesenau pig model of streptozotocin-induced diabetes is an important task, since this breed has not yet been used, and it is also one of the most accessible breeds of laboratory pigs in the world. Russia. AIM: To reproduce the model of streptozotocin-induced diabetes in Wiesenau pigs with correction of hyperglycemia by insulin therapy and evaluate its stability and compliance with the experimental model of type 1 diabetes. MATERIALS AND METHODS: The study was performed on 6 Wiesenau pigs. Diabetes was induced by intravenous administration of streptozotocin (150 mg/kg). Blood glucose concentration was determined with a glucometer and a continuous glucose monitoring system was used. The content of insulin in the blood was determined by enzyme-linked immunosorbent assay (Cloud-clone corp). The success of diabetes induction was confirmed by a glucose tolerance test. Seven days after the injection of STZ, insulin therapy was initiated and continued for three months. Insulin therapy was carried out for three months. 3 months after induction, a histological and immunohistochemical study of the condition of the pancreatic islets was carried out. RESULTS: A study in Wiesenau pigs showed that a dose of 150 mg/kg streptozotocin was safe and effective in inducing diabetes in 67% of cases. An optimal individual insulin therapy protocol was selected, which successfully reduces blood glucose levels to normal values. This model of streptozotocin-induced diabetes in Wiesenau pigs is stable for 90 days and can be used to study new antidiabetic agents. CONCLUSION: A model of STZ-induced diabetes has been established in Wiesenau pigs. The stability of the model has been confirmed under conditions of glucose toxicity alleviation through insulin therapy.