What does this research mean for the field?

Nitric oxide delays carotenoid accumulation in tomato fruit by suppressing the transcriptional activity of SlbZIP16, which subsequently inhibits the activation of the SlPol η module. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To elucidate the molecular mechanism by which nitric oxide suppresses carotenoid biosynthesis and delays color development during tomato fruit ripening.

August 24, 2026Plant Physiology and BiochemistryOpen Access

Nitric oxide delays tomato fruit carotenoid accumulation by inhibiting the transcriptional activation of the SlbZIP16-SlPol η module

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Authors

YLYaling Li DHDandan Huang SZShuhua Zhu

Discussion

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Overview

Experimental study reveals that nitric oxide suppresses the SlbZIP16-SlPol η pathway in tomatoes, suggesting a targeted mechanism to regulate fruit pigmentation and ripening.

Key Points

To elucidate the molecular mechanism by which nitric oxide suppresses carotenoid biosynthesis and delays color development during tomato fruit ripening.
Assessed SlPol η function in tomato fruit using transient overexpression and virus-induced gene silencing (VIGS).
Characterized SlbZIP16 interaction with the SlPol η promoter using yeast one-hybrid, electrophoretic mobility shift assays (EMSA), dual-luciferase reporter, and GUS assays.
Evaluated the effect of exogenous nitric oxide and performed density functional theory (DFT) calculations to model SlbZIP16 nitration.
SlPol η overexpression upregulated carotenoid biosynthesis genes and accelerated chlorophyll degradation, whereas VIGS silencing markedly delayed fruit coloring.
SlbZIP16 directly bound to and activated the SlPol η promoter, but exogenous nitric oxide significantly suppressed SlbZIP16 transcriptional activity and delayed ripening.
DFT modeling identified Tyr173 of SlbZIP16 as a high-confidence target for nitric-oxide-mediated nitration, predicted to cause conformational disturbances and impair protein function.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/6a8c005bbca056c88e6deffb https://doi.org/10.1016/j.plaphy.2026.111678

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August 24, 2026Plant Physiology and BiochemistryOpen Access

Nitric oxide delays tomato fruit carotenoid accumulation by inhibiting the transcriptional activation of the SlbZIP16-SlPol η module

View Full Paper

Ask AI

Bookmark

Authors

YLYaling Li DHDandan Huang SZShuhua Zhu

Discussion

Member takes

Overview

Experimental study reveals that nitric oxide suppresses the SlbZIP16-SlPol η pathway in tomatoes, suggesting a targeted mechanism to regulate fruit pigmentation and ripening.

Key Points

To elucidate the molecular mechanism by which nitric oxide suppresses carotenoid biosynthesis and delays color development during tomato fruit ripening.
Assessed SlPol η function in tomato fruit using transient overexpression and virus-induced gene silencing (VIGS).
Characterized SlbZIP16 interaction with the SlPol η promoter using yeast one-hybrid, electrophoretic mobility shift assays (EMSA), dual-luciferase reporter, and GUS assays.
Evaluated the effect of exogenous nitric oxide and performed density functional theory (DFT) calculations to model SlbZIP16 nitration.
SlPol η overexpression upregulated carotenoid biosynthesis genes and accelerated chlorophyll degradation, whereas VIGS silencing markedly delayed fruit coloring.
SlbZIP16 directly bound to and activated the SlPol η promoter, but exogenous nitric oxide significantly suppressed SlbZIP16 transcriptional activity and delayed ripening.
DFT modeling identified Tyr173 of SlbZIP16 as a high-confidence target for nitric-oxide-mediated nitration, predicted to cause conformational disturbances and impair protein function.

Cite This Study

Li et al. (2026) studied this question.

synapsesocial.com/papers/6a8c005bbca056c88e6deffb https://doi.org/10.1016/j.plaphy.2026.111678

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