Influence of Scattering on Fluorescence Spectra of Dilute Solutions Obtained with the Aminco–Bowman Spectrophotofluorometer

Various factors affecting the intensity of scattered radiation appearing in fluorescence and excitation spectra of molecules in dilute solution obtained with the Aminco–Bowman Spectrophotofluorometer have been investigated. The spectra can be modified and in some cases improved by the addition of colored glass filters, interference filters, and polarizing elements to the optical system. The polarizing elements permit the measurement of fluorescence polarization, and results are given for the polarization of fluorescence of some xanthydrol derivatives in glycerol at room temperature. The effects of strong sample absorption and highly intense scattered radiation on observed fluorescence spectra and quantitative assays are shown.