Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

This is the authors' abstract. We don't add key points for this paper.

Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.