A method for determining the pH of small quantities of fluid

Ann Bishop; E. F. Hartree; Elspeth W. McConnachie

doi:10.1017/s0031182000026445

May 1, 1956Parasitology

A method for determining the pH of small quantities of fluid

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Key Points

To develop a modified micro-glass electrode capable of measuring the pH of minute fluid volumes using a standard pH meter.
Constructed a micro-electrode from Corning 015 glass capillary tubing with an outer diameter of 0.5–0.75 mm and length of approximately 6 cm.
Drew the capillary walls extremely thin to keep total electrode electrical resistance beneath the 500 MΩ threshold required by the Pye Universal pH meter.
Successfully adapted Caldwell's micro-electrode configuration to operate without an electrometer impedance converter circuit.
Maintained electrode resistance below 500 MΩ, enabling direct and accurate pH measurements of small blood volumes extracted from mosquito stomachs.

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Implication

Experimental study demonstrates a modified micro-glass electrode for measuring blood pH in mosquito stomachs, suggesting broad utility for testing minute fluid samples.

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Cite This Study

Bishop et al. (1956) studied this question.

synapsesocial.com/papers/6a8c108f3c68152a7ce97d73 https://doi.org/10.1017/s0031182000026445

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A method for determining the pH of small quantities of fluid

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A method for determining the pH of small quantities of fluid

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