2′-Deoxy-2′-fluorouridine-5′-triphosphates: a possible substrate for E. coli RNA polymerase

dUflTP was tested as substrate in the E. coli RNA polymerase system using poly(dAT) as template. dUflTP could replace UTP when Mn++ was utilized as divalent cation instead of Mg++. The level of transcription with the fluoro analog was then 55% of that with UTP.