What question did this study set out to answer?

To investigate the role of transforming growth factor beta (TGF-beta) in regulating the pathogenesis, replication, and latency of Leishmania braziliensis infections.

April 15, 1993Proceedings of the National Academy of SciencesOpen Access

Transforming growth factor beta as a virulence mechanism for Leishmania braziliensis.

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Authors

ABAldina BarralInstituto Federal da Bahia MBManoel Barral‐NettoUniversidade Federal da Bahia EYE C YongUniversity of Washington

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Implication

Preclinical study demonstrates that transforming growth factor beta exacerbates Leishmania braziliensis infection in murine models, highlighting cytokine-mediated parasite virulence.

Key Points

To investigate the role of transforming growth factor beta (TGF-beta) in regulating the pathogenesis, replication, and latency of Leishmania braziliensis infections.
Inoculated murine peritoneal macrophage cultures with L. braziliensis in the presence of recombinant TGF-beta or neutralizing anti-TGF-beta monoclonal antibodies to assess intracellular replication and active TGF-beta output.
Administered recombinant TGF-beta to mice infected 15 weeks prior with virulent or avirulent L. braziliensis isolates to evaluate reactivation of latent infection and quantify interleukin 10 mRNA expression in draining lymph nodes.
Recombinant TGF-beta increased intracellular L. braziliensis replication in macrophage cultures, whereas neutralizing anti-TGF-beta monoclonal antibodies reduced parasite load.
Infected macrophages produced biologically active TGF-beta in direct proportion to the intrinsic virulence of the parasite isolates.
In vivo recombinant TGF-beta administration converted avirulent parasites into virulent forms, reactivated latent infections in healed or uninjured mice at 15 weeks post-infection, and upregulated interleukin 10 mRNA in draining lymph nodes.

Cite This Study

Barral et al. (1993) studied this question.

synapsesocial.com/papers/6a8c27d3fe910689a2894592 https://doi.org/10.1073/pnas.90.8.3442

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Implication

Preclinical study demonstrates that transforming growth factor beta exacerbates Leishmania braziliensis infection in murine models, highlighting cytokine-mediated parasite virulence.

Transforming growth factor beta as a virulence mechanism for Leishmania braziliensis.

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Implication

Key Points

Cite This Study

Transforming growth factor beta as a virulence mechanism for Leishmania braziliensis.

Discussion

Member takes

Implication

Key Points

Cite This Study

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