Metabolic Reprogramming and Inflammatory Response Induced by D-Lactate in Bovine Fibroblast-Like Synoviocytes Depends on HIF-1 Activity

Figures

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  Figure 1. Metabolomic profile of bovine fibroblast-like synoviocytes (bFLS) after D-lactate treatment. (A) Hierarchical clustering analysis of 50 metabolites from the control untreated and 5 mM D-lactate-stimulated bFLS. The red and blue colors indicate that the metabolite level is increased and decreased compared to the mean metabolite relative abundance, respectively. Each column represents a sample clustered according to treatment, and each row represents an individual metabolite. n = 4. (B) The partial least squares-discriminant analysis (PLS-DA) score plot based on metabolomic analysis of D-lactate stimulated (green) and control (red) bFLS. The explained variances of the selected components are shown in brackets. n = 4.

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  Figure 2. D-Lactate alters the intracellular concentration of several metabolites. The concentrations of metabolites significantly altered after 1 h stimulation with 5 mM D-lactate are expressed as relative abundance with respect to ribitol. Each bar represents the mean ± SEM. Each point represents an independent experiment, n = 4. *p < 0.05.

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  Figure 3. D-Lactate treatment increases the intracellular levels of both lactate stereoisomers as well as the L-lactate dehydrogenase subunit A (L-LDHA) expression. bFLS were treated with 5 mM D-lactate for 1 h. (A) L-lactate and (B) D-lactate were quantified at the intracellular level by high-performance liquid chromatography (HPLC). Each bar represents the mean ± SEM. Each point represents an independent experiment, n = 5. (C) The relative mRNA expression of L-LDHA was assessed by RT-qPCR. Bovine tumore necrosis factor-α (TNF-α) was used as the positive control. Each bar represents the mean ± SEM, n = 5. *p < 0.05; **p < 0.01.

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  Figure 4. D-Lactate reprograms bFLS metabolism. bFLS were stimulated with 5 mM D-lactate for 1 h. Metabolic pathway analysis was performed using MetaboAnalyst v4.0 based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) using all metabolites significantly altered after D-lactate treatment. All matched pathways are shown as circles. The color of the circles is based on p-values from pathway enrichment analysis, where darker colors indicate more significant metabolites changes in the corresponding pathway. The size of the circles represents the pathway impact score. The most impacted pathways with high statistical significance (p < 0.05) are labeled. n = 4.

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  Figure 5. D-Lactate increases the expression of inflammation and metabolism-associated genes in bFLS. Relative mRNA expression of (A) IL-6, (B) hypoxia-inducible factor-1 subunit α (HIF-1α), (C) glucose transporter 1 (Glut-1), and (D) pyruvate dehydrogenase kinase 1 (PDK-1) in bFLS stimulated with 5 mM D-lactate for 6 h. bTNF-α was used as the postitive control. Each bar represents the mean ± SEM, n = 5. *p < 0.05; **p < 0.01.

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  Figure 6. D-Lactate induces HIF-1α protein accumulation under normoxic conditions in bFLS. bFLS were stimulated with 5 mM D-lactate for 6 h under normoxic (20% O2) and hypoxic (1% O2) conditions. bTNF-α was used as the positive control. HIF-1α stabilization was detected in total protein extracts by Western blot using cobalt chloride (CoCl2) as the hypoxia-mimetic agent. (A) Representative HIF-1α immunoblot is shown. (B) Densitometry HIF-1α values were quantified using Image Studio Lite v5.2 software and normalized to β-actin. Each bar represents the mean ± SEM, n = 3. **p < 0.01; ****p < 0.0001; n.s., not significant.

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  Figure 7. HIF-1 is involved in the D-lactate-induced overexpression of inflammation- and metabolism-associated genes in bFLS. bFLS were preincubated with 40 μM YC-1 and then stimulated with 5 mM D-lactate for 6 h. The relative mRNA expression levels of (A) IL-6, (C) HIF-1α, (D) Glut-1, and (E) PDK-1 are shown. (B) IL-6 levels in conditioned media were measured by ELISA. bTNF-α was used as the positive control. Each bar represents the mean ± SEM, n = 5. *p < 0.05; **p < 0.01; n.s., not significant.

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  Figure 8. The PI3K/Akt pathway and the NF-κB pathway regulate the overexpression of metabolic genes induced by D-lactate in bFLS. bFLS were preincubated with 10 μM LY294002 or 10 μM BAY 11-7082 and stimulated with 5 mM D-lactate for 6 h. The relative mRNA expression levels of (A,B) HIF-1α, (C,D) Glut-1, and (E,F) PDK-1 are shown. bTNF-α was used as positive control. Each bar represents the mean ± SEM, n = 5. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

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  Figure 9. Metabolic reprogramming supports the inflammatory response induced by D-lactate in bFLS. D-Lactate induces the PI3K/Akt pathway activation and downstream activation of NF-κB and HIF-1. Through this signaling pathway, D-lactate induces the gene expression of IL-6, with the pro-inflammatory cytokine involved in the synovial inflammatory response. HIF-1 activation also increases the expression of the Glut-1 transporter, which increases glucose uptake for use in glycolysis. Glycolysis is also favored by the HIF-1-dependent overexpression of L-LDH, which oxidizes pyruvate to L-lactate. In addition, an increased PDK-1 expression blocks the mitochondrial utilization of pyruvate through the TCA cycle, contributing to the glycolytic fate of glucose. Overexpression of the HIF-1α subunit would favor the accumulation of HIF-1 heterodimers, maintaining glycolytic metabolic reprogramming. IL-6, interleukin 6; Glut-1, solute carrier family 2 (facilitated glucose transporter) member 1; L-LDH, L-lactate dehydrogenase; PDK-1, pyruvate dehydrogenase kinase 1; HIF-1α, hypoxia inducible factor 1 subunit alpha; PDH, pyruvate dehydrogenase; TCA, tricarboxylic acid cycle; PI3K, phosphatidyl inositol…