Effect of Hormones on the in Vitro Incorporation of Glycine-1-C14 Into Reticulocyte Proteins1

IT IS known that many steroid hormones affect both protein metabolism (Rutgers University Protein Symposium, 1953) and the formed elements of the blood (Gordon, 1954). Thus, the administration of ACTH results in an increase in urinary amino acids (Ronzoni et al., 1953) and adrenalectomy of the eviscerated rat causes a decrease of plasma amino acids (Ingle et al., 1950). Barton and Rusch (1951) have shown that administration of adrenocortical extract inhibited the in vivo incorporation of glycine-2-C14 into several tissue proteins. Similarly, it has been found by Sinex (1954) that prior treatment of intact rats with ACTH will cause a decrease in the in vitro incorporation of alanine-1-C14 into liver slices.