August 1, 1991AJP Gastrointestinal and Liver Physiology

Kinetics and regulation of a polarized Na(+)-H+ exchanger from Caco-2 cells, a human intestinal cell line

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Population

Caco-2 cells (human intestinal cell line) grown on membrane filters

Comparison

Exposure to forskolin, 1,9-dideoxyforskolin… vs Basal conditions

Design

Preclinical

Authors

AWAngela WatsonQueensland University of Technology SLS. A. LevineCentre National de la Recherche Scientifique MDMark DonowitzJohns Hopkins University

Discussion

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Implication

May refine epithelial pH models; leaves open in vivo confirmation and clinical relevance.

Structured PICO

Population

Caco-2 cells (human intestinal cell line) grown on membrane filters

Intervention

Exposure to forskolin, 1,9-dideoxyforskolin, 8-BrcAMP, E. coli STa toxin, ionomycin, phorbol dibutyrate, or cellular shrinkage in hypertonic medium

Comparator

Basal conditions

Outcome

Na(+)-H+ exchange rate (measured as Na(+)-dependent H+ efflux in response to an acid load)surrogate

In Caco-2 cells, the basolateral Na(+)-H+ exchanger regulates intracellular pH but is uniquely unresponsive to typical second messenger pathways and cell shrinkage.

Cite This Study

Watson et al. (1991) studied this question.

synapsesocial.com/papers/6a8c2b56eada60f85de3dcfa https://doi.org/10.1152/ajpgi.1991.261.2.g229

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Kinetics and regulation of a polarized Na(+)-H+ exchanger from Caco-2 cells, a human intestinal cell line

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Kinetics and regulation of a polarized Na(+)-H+ exchanger from Caco-2 cells, a human intestinal cell line

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider