Key result
Primary cultures from fetal rat brain synthesize an angiotensin II-like immunoreactivity, and this synthesis is significantly inhibited by Captopril.
Demonstrates that primary cultures from fetal rat brain can synthesize angiotensin II-like immunoreactivity, supporting local brain synthesis.
Suggests local angiotensin II synthesis in fetal rat brain; leaves open relevance to adult human cardiovascular regulation.
Primary cultures from fetal rat brain contain angiotensin II immunoreactivity in neurons. To study the origin of this immunoreactivity, primary cultures were incubated with [3H]-valine or [3H]-isoleucine. A time-dependent incorporation of radioactivity into immunoprecipitable angiotensin II was observed. Incubation of cultures with Captopril, an inhibitor of the converting enzyme, resulted in a significant inhibition of [3H]-valine incorporation into immunoreactive [3H]-angiotensin II. These results demonstrate that primary cultures from fetal rat brain can synthesize an angiotensin II-like immunoreactivity and support a concept that angiotensin II may be synthesized in fetal rat brain.
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Raizada et al. (2008) studied this question. [3H]-valine or [3H]-isoleucine and Captopril was evaluated on Incorporation of radioactivity into immunoprecipitable angiotensin II. Primary cultures from fetal rat brain synthesize an angiotensin II-like immunoreactivity, and this synthesis is significantly inhibited by Captopril.
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