Epidemiological Surveillance of Norovirus and Rotavirus in Sewage (2016–2017) in Valencia (Spain)

Figures

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  Figure 1. Overview of the concentrations and genotypes of enteric viruses detected in sewage. Boxplots show median concentrations (log PCRU/L) with the 25th and 75th percentile values of norovirus GI (A panel in dark blue), norovirus GII (B panel in light blue), and rotavirus (C panel in green). Viral genotypes are indicated according to the month in which the sample was sampled.

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  Figure 2. Molecular phylogenetic analysis of norovirus GI capsid. The evolutionary history was inferred using the Maximum Likelihood method based on the Kimura 2-parameter model [42] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 18 nucleotide sequences. There were 276 positions in the final dataset including nucleotides from 5386 to 5662.

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  Figure 3. Molecular phylogenetic analysis of norovirus GII capsid. The evolutionary history was inferred by using the Maximum Likelihood method based on the Kimura 2-parameter model [42] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 43 nucleotide sequences. There were 273 positions in the final dataset including nucleotides from 5113 to 5386. a, b, and c: Boxes that contain identical sequences.

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  Figure 4. Molecular phylogenetic analysis of P[8] rotavirus (VP4 gene). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model [43] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 32 nucleotide sequences. There were 127 positions in the final dataset. including nucleotides from 180 to 307. a, b, and c: Boxes containing identical sequences.

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  Figure 5. Molecular phylogenetic analysis of G1 rotavirus (VP7 gene). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model [43] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 16 nucleotide sequences. There were 416 positions in the final dataset including nucleotides from 358 to 774.

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  Figure 6. Molecular phylogenetic analysis of G2 rotavirus (VP7 gene). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model [43] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 18 nucleotide sequences. There was a total of 460 positions in the final dataset including nucleotides from 424 to 884.

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  Figure 7. Molecular phylogenetic analysis of G3 rotavirus (VP7 gene). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model [43] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 10 nucleotide sequences. There was a total of 159 positions in the final dataset including nucleotides from 774 to 933.

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  Figure 8. Molecular phylogenetic analysis of G9 rotavirus (VP7 gene). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model [43] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis included 11 nucleotide sequences. There was a total of 159 positions in the final dataset including nucleotides from 774 to 933.

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  Figure 9. Molecular phylogenetic analysis of G12 rotavirus (VP7 gene). The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura 3-parameter model [43] with a bootstrap of 1000 replicates. The tree is drawn to scale, the branch lengths measure the number of substitutions per site. The analysis involved 16 nucleotide sequences. There was a total of 378 positions in the final dataset including nucleotides from 584 to 926.