Key result
Rotavirus A and human adenovirus were detected in 90% and 100% of wastewater samples respectively, highlighting the utility of environmental surveillance for monitoring viral epidemiology.
Why the study?
Rotavirus A is a major public health threat and cause of infantile gastroenteritis that persists in the environment, prompting surveillance in wastewater matrices.
Observational
Yes
Monitoring water matrices for RVA epidemiology is a useful tool to evaluate and predict possible emergence or reemergence of uncommon strains in a region.
Affirms wastewater surveillance utility for RVA/HAdV; leaves open ties to clinical gastroenteritis burden.
Rotavirus A (RVA) is the most common virus associated with infantile gastroenteritisworldwide, being a public health threat, as it is excreted in large amounts in stool and can persist inthe environment for extended periods. In this study, we performed the detection of RVA and humanadenovirus (HAdV) by TaqMan qPCR and assessed the circulation of RVA genotypes in threewastewater treatment plants (WWTPs) between 2015 and 2016 in Catalonia, Spain. RVA wasdetected in 90% and HAdV in 100% of the WWTP samples, with viral loads ranging between 3.96 ×104 and 3.30 × 108 RT-PCR Units/L and 9.51 × 104 and 1.16 × 106 genomic copies/L, respectively. RVAVP7 and VP4 gene analysis revealed the circulation of G2, G3, G9, G12, P[4], P[8], P[9] and P[10].Nucleotide sequencing (VP6 fragment) showed the circulation of I1 and I2 genotypes, commonlyassociated with human, bovine and porcine strains. It is important to mention that the RVA strainsisolated from the WWTPs were different from those recovered from piglets and calves living in thesame area of single sampling in 2016. These data highlight the importance of monitoring watermatrices for RVA epidemiology and may be a useful tool to evaluate and predict possibleemergence/reemergence of uncommon strains in a region.
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Silva‐Sales et al. (2020) conducted an observational in Rotavirus A and human adenovirus in wastewater. Wastewater surveillance for RVA and HAdV was evaluated on Detection rate of RVA and HAdV. Rotavirus A and human adenovirus were detected in 90% and 100% of wastewater samples respectively, highlighting the utility of environmental surveillance for monitoring viral epidemiology.
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