What question did this study set out to answer?

To isolate and characterize the serum factor from cystic fibrosis patients and heterozygote carriers that inhibits ciliary movement in oyster gills.

February 6, 1970Science

Cystic Fibrosis: Characterization of the Inhibitor to Ciliary Action in Oyster Gills

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Biochemical analysis reveals an immunoglobulin G-associated ciliary inhibitor in cystic fibrosis patients, indicating a distinctive serum factor.

To isolate and characterize the serum factor from cystic fibrosis patients and heterozygote carriers that inhibits ciliary movement in oyster gills.
Fractionated serum collected from cystic fibrosis patients, heterozygotes, and healthy controls.
Characterized the inhibitory component using electrophoresis, gel filtration, and diethylaminoethyl-cellulose chromatography.
Assayed the biological activity of the isolated fractions against oyster gill ciliary motility.
The ciliary inhibitor was isolated as a cationic protein fraction with an estimated molecular weight between 125,000 and 200,000.
The active inhibitory factor co-eluted with the immunoglobulin G fraction during diethylaminoethyl-cellulose chromatography.
Equivalent serum fractions derived from healthy control individuals produced no inhibition of oyster ciliary activity.

Bowman et al. (1970) studied this question.

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Biochemical analysis reveals an immunoglobulin G-associated ciliary inhibitor in cystic fibrosis patients, indicating a distinctive serum factor.