In studying the phosphorous metabolism of dairy cows by the use of radioactive tracers (i6), it was necessary to develop a suitable injection and bleeding technique which would fulfill the following requirements: (a) It must permit complete injection of the radioactive material for hazard control; (b) it must provide for accurate and rapid injection of the material, followed immediately with the withdrawal of blood samples; (c) it must allow for the bleeding at frequent intervals over long periods (4 to 5 weeks) ; and (d) it must minimize the disturbance and apprehension of the cow, conditions which have been shown to affect the blood phosphorous values.
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Ralston et al. (1949) studied this question.
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