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An analysis is made of environmental factors controlling growth rate and maximum population density of cultured mammalian cells. Three factors may limit maximum population density: accumulation of an inhibitor, exhaustion of essential nutrients or availability of surface for growth. Whilst the significance of inhibitors is uncertain, examples are given of nutrient exhaustion limiting maximum population density. Growth of anchorage‐dependent cells is influenced by both available surface area and by the physicochemical properties of the surface. Finally examples are given of ways in which environmental terms may interact, often in complex fashion, to modify cell growth.
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Birch et al. (1982) studied this question.
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