May 1, 1993AJP Cell Physiology

Use of a PCR-based method to characterize protein kinase C isoform expression in cardiac cells

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Population

Adult rat cardiac myocytes and cultured neonatal ventricular myocytes (hippocampus used for validation)

Design

Preclinical

Authors

TKTrudy A. KohoutUniversity of North Carolina at Chapel Hill TRT B RogersUniversity of Maryland, Baltimore

Discussion

Member takes

Implication

RT-PCR detects PKC isoforms in rat myocytes; extends isoform mapping but leaves open human cardiac relevance.

Structured PICO

Population

Adult rat cardiac myocytes and cultured neonatal ventricular myocytes (hippocampus used for validation)

Intervention

Reverse transcriptase-polymerase chain reaction (RT-PCR) using degenerate oligonucleotide primers

Outcome

Characterization of protein kinase C (PKC) isoform expression

Development of an RT-PCR method allowed for the efficient characterization of PKC isoform diversity in cardiac cells, identifying alpha, delta, epsilon, eta, and zeta isoforms.

Cite This Study

Kohout et al. (1993) studied this question.

synapsesocial.com/papers/6a8c8a41735d7c8232130dce https://doi.org/10.1152/ajpcell.1993.264.5.c1350

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May 1, 1993AJP Cell Physiology

Use of a PCR-based method to characterize protein kinase C isoform expression in cardiac cells

View Full Paper

Ask AI

Bookmark

Population

Adult rat cardiac myocytes and cultured neonatal ventricular myocytes (hippocampus used for validation)

Design

Preclinical

Authors

TKTrudy A. KohoutUniversity of North Carolina at Chapel Hill TRT B RogersUniversity of Maryland, Baltimore

Discussion

Member takes

Implication

RT-PCR detects PKC isoforms in rat myocytes; extends isoform mapping but leaves open human cardiac relevance.

Structured PICO

Population

Adult rat cardiac myocytes and cultured neonatal ventricular myocytes (hippocampus used for validation)

Intervention

Reverse transcriptase-polymerase chain reaction (RT-PCR) using degenerate oligonucleotide primers

Outcome

Characterization of protein kinase C (PKC) isoform expression

Development of an RT-PCR method allowed for the efficient characterization of PKC isoform diversity in cardiac cells, identifying alpha, delta, epsilon, eta, and zeta isoforms.

Cite This Study

Kohout et al. (1993) studied this question.

synapsesocial.com/papers/6a8c8a41735d7c8232130dce https://doi.org/10.1152/ajpcell.1993.264.5.c1350

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