A Technique for Estimation of Platelet-Collagen Adhesion

A technique is described which measures the adhesion of platelets to connective tissue fragments in platelet-rich plasma (PRP). The principle of the method is measurement of OD in EDTA–PRP to which connective tissue fragments have been added. A fall in OD occurs as the platelets line up on connective tissue fibers. Aspirintreated platelets adhere normally, although they show reduced aggregation with collagen in citrated PRP. An additional observation is that in the presence of sufficient connective tissue, all platelets participate in the adhesion reaction.