Rat insulin-degrading enzyme (IDE) binds atrial natriuretic peptide (ANP) with high affinity, suggesting a potential role in its cellular processing or metabolic clearance.
IDE-ANP binding in rats suggests a clearance role; leaves open relevance to human cardiovascular regulation.
A cytosolic protein specifically binding to and degrading atrial natriuretic peptide (ANP) was purified from rat brain homogenate. Based on partial amino acid sequences and enzymatic properties, this protein with an apparent molecular mass of 112 kDa has been identified as the rat insulin-degrading enzyme (IDE). In addition to the known substrates, insulin and transforming-growth-factor alpha IDE binds also with high affinity (apparent Kd 60 nM) to ANP. Competition studies with structural variants of ANP demonstrate that both the C terminus and the disulfide loop of the molecule are essential for high-affinity binding. The data suggest that IDE might be involved in the cellular processing and/or metabolic clearance of ANP.
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Müller et al. (1991) studied this question.
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