Discovery and Target Identification of an Antiproliferative Agent in Live Cells Using Fluorescence Difference in Two‐Dimensional Gel Electrophoresis

Target acquired: Fluorescence difference in two-dimensional gel electrophoresis (FITGE) was developed to observe the interactions between proteins and small molecules in an intact cellular environment. FITGE proved effective over conventional methods by successfully identifying the protein target of an anti-proliferative compound in live cells through the differentiation between specific and extensive non-specific binding of photoaffinity probes.