Phospholipase A activity was demonstrated in guinea pig spermatozoa using [U-14C] phosphatidyl choline as a substrate. The activity had a neutral pH optimum, was stimulated by Ca2+ and low concentrations of detergent, and. was inhibited by EDTA, mepacrine and p-bromophenacyl bromide. Appropriate concentrations of mepacrine and p-bromophenacyl bromide inhibited the acrosome reactions of capacitated spermatozoa without interfering with their motility. These results support the notion that phospholipase A is involved in the acrosome reaction of mammalian spermatozoa.
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Ono et al. (1982) studied this question.
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