Respiratory activity of ejaculated bovine semen was studied in the presence of phosphate buffer (pH 7·4), egg-yolk medium or media containing different fractions isolated from the egg-yolk. Seminal plasma from centrifuged bull semen has very little respiratory activity as compared with whole semen. The main factor affecting the respiratory activity of the semen is the concentration of active spermatozoa, but the respiratory activity is not directly proportional to the concentration of spermatozoa, since it falls off with reduction of concentration.
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Tošić et al. (1947) studied this question.
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