What question did this study set out to answer?

To evaluate the phosphorylation dynamics of eukaryotic initiation factor 4E (eIF-4E) and its link to protein biosynthesis during resting B cell activation.

November 1, 1990Journal of Biological ChemistryOpen Access

Increased rate of phosphorylation-dephosphorylation of the translational initiation factor eIF-4E correlates with the induction of protein and glycoprotein biosynthesis in activated B lymphocytes.

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To evaluate the phosphorylation dynamics of eukaryotic initiation factor 4E (eIF-4E) and its link to protein biosynthesis during resting B cell activation.
Stimulated resting murine splenic B lymphocytes with bacterial lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA) plus ionomycin.
Measured eIF-4E turnover rates using metabolic 32Pi labeling and evaluated pathway mechanisms with protein kinase inhibitors (H-7, HA1004) and okadaic acid.
Cell activation induced a 10- to 50-fold biphasic increase in eIF-4E 32Pi labeling, whereas the phosphorylated fraction increased from 46% to 83% in LPS-activated cells.
The eIF-4E phosphate group displayed a rapid turnover half-life of 2 hours, whereas the eIF-4E polypeptide chain demonstrated no detectable turnover within 6 hours.
eIF-4E phosphorylation was insensitive to kinase inhibitors H-7 and HA1004, while in vivo dephosphorylation was inhibited by okadaic acid with an IC50 of 200 nM.

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Implication

Laboratory study demonstrates rapid eIF-4E phosphorylation-dephosphorylation in activated B cells, suggesting translational control via protein phosphatase-1.

Cite This Study

Rychlik et al. (1990) studied this question.

synapsesocial.com/papers/6a8d061143002c564690652f https://doi.org/10.1016/s0021-9258(17)45396-8

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Implication

Laboratory study demonstrates rapid eIF-4E phosphorylation-dephosphorylation in activated B cells, suggesting translational control via protein phosphatase-1.

Increased rate of phosphorylation-dephosphorylation of the translational initiation factor eIF-4E correlates with the induction of protein and glycoprotein biosynthesis in activated B lymphocytes.

Key Points

Discussion

Implication

Cite This Study

Increased rate of phosphorylation-dephosphorylation of the translational initiation factor eIF-4E correlates with the induction of protein and glycoprotein biosynthesis in activated B lymphocytes.

Key Points

Discussion

Implication

Cite This Study

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