VISIBLE ABSORPTION SPECTRA OF CHLOROPHYLL b IN METHANOL, DIOXANE AND/OR WATER SOLUTIONS

Chlorophyll b (Chl-b) in methanol solution absorbing at 650 nm (Cb65O) forms microcrystals absorbing at 680 nm after the addition of water. In methanol-dioxane-water solutions. Chl-b molecules form an aggregate absorbing at 672 nm (Cb672). Cb672 was obtained either by mixing a small volume of a methanol-dioxane solution of Chl-b with a large volume of water, or by mixing a large volume of methanol-water solution of the pigment with a small volume of dioxane.