Characterization of Lachnoanaerobaculum sanguinis sp. nov., isolated from a blood culture of an acute myeloid leukemia patient with chemotherapy-related bacteremia

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This study was to characterize and determine the classification of the novel strain TM49 T , isolated from a blood culture of a patient who developed bacteremia while receiving high-dose cytarabine chemotherapy for acute myeloid leukemia. The novel strain TM49 T was characterized at the species level through a polyphasic taxonomic analysis, incorporating morphological, physiological, biochemical, phylogenetic, and genome sequence analyses. 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Lachnoanaerobaculum . The 16S rRNA gene sequence of strain TM49 T was observed to be most similar to that of " Lachnoanaerobaculum gingivalis " strain ChDC B114 T (99.2 %). Strain TM49 T formed circular, glossy, slightly milky colonies with an undulate margin on BD™ Columbia Agar containing 5 % sheep blood after 48 h at 37 °C, exhibiting less spreading compared to species of the genus Lachnoanaerobaculum . The major fatty acids of strain TM49 T were C 16:0 , C 14:0 , C 18:1 ω9c DMA, and C 18:1 ω7c DMA. The draft genome sequence of strain TM49 T was 2,821,554 bp in length, with a G + C content of 36.4. Digital DNA-DNA hybridization values between strain TM49 T and “ L. gingivalis ” ChDC B114 T , L. umeaense CD3: 22 T , L. orale N1 T , and L. saburreum CCUG 28089 T were 52.2 %, 29.7 %, 26.2 %, and 24.5 %, respectively. The Orthologous Average Nucleotide Identity values between strain TM49 T and “ L. gingivalis ” ChDC B114 T , L. umeaense CD3: 22 T , L. orale N1 T , and L. saburreum CCUG 28089 T were 93.1 %, 84.9 %, 83.2 %, and 81.5 %, respectively. Strain TM49 T (JCM 36186 T = DSM 116945 T ) was found to be distinct from previously described species of the genus Lachnoanaerobaculum . Therefore, it is proposed as a new species, Lachnoanaerobaculum sanguinis sp. Nov., with strain TM49 T as the type strain (= JCM 36186 T = DSM 116945 T ). • Strain TM49 T was isolated from a blood culture of an acute myeloid leukemia patient with bacteremia duringchemotherapy. • 16S rRNA gene sequence analysis identified it as belonging to the genus Lachnoanaerobaculum. • The draft genome of TM49 T is 2,821,554 bp with a G + C content of 36.4 mol%. • DNA-DNA hybridization and OrthoANI analysis confirmed it as a novel species. • TM49 T is proposed as Lachnoanaerobaculum sanguinis sp. Nov.