What question did this study set out to answer?

To determine the immediate and long-term metabolic fate, chemical interconversion, and tissue reutilization of injected 75Se-selenomethionine in rats.

February 1, 1967Journal of Biological ChemistryOpen Access

The Interconversion and Reutilization of Injected 75Se-Selenomethionine in the Rat

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Authors

HAHassan K. AwwadBrigham and Women's Hospital SAS. James AdelsteinUniversity of Massachusetts Boston EPE. James PotchenOffice of Education

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Implication

Animal study demonstrates the metabolic conversion and efficient reutilization of injected selenomethionine into selenocysteine analogues in rats, indicating tight recycling of selenoamino acids.

Key Points

To determine the immediate and long-term metabolic fate, chemical interconversion, and tissue reutilization of injected 75Se-selenomethionine in rats.
Injected rats with 75Se-selenomethionine to monitor downstream tracer dynamics.
Isolated the liver precursor amino acid pool, liver protein hydrolysates, and serum albumin.
Identified radioseleno compounds and measured specific activity over time using two-solvent chromatographic separation.
Detected two radioseleno metabolites in hepatic precursor pools and protein hydrolysates with chromatographic mobility matching L-cysteine and L-cystine.
Demonstrated that the specific activity kinetics of L-methionine and L-cysteine/L-cystine analogues follow a classic precursor-product relationship.
Derived serum albumin synthesis rates that align with known rat turnover rates and confirmed the efficient metabolic reutilization of selenoamino acids.

Cite This Study

Awwad et al. (1967) studied this question.

synapsesocial.com/papers/6a8d3a8ba4bc1cf4cf7aa984 https://doi.org/10.1016/s0021-9258(18)96300-3

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Implication

Animal study demonstrates the metabolic conversion and efficient reutilization of injected selenomethionine into selenocysteine analogues in rats, indicating tight recycling of selenoamino acids.

The Interconversion and Reutilization of Injected 75Se-Selenomethionine in the Rat

Discussion

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Implication

Key Points

Cite This Study

The Interconversion and Reutilization of Injected 75Se-Selenomethionine in the Rat

Discussion

Member takes

Implication

Key Points

Cite This Study

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