Key result
A novel pseudovirus system for human Aichi virus was successfully developed, demonstrating high susceptibility in HeLa cells and enabling a bioluminescent imaging infection model in BALB/c mice.
Why the study?
Human Aichi virus has been described as a causative agent of gastroenteritis, but research on it at cellular and animal levels is limited.
The developed AiV pseudovirus system successfully infects HeLa cells and BALB/c mice, providing a safe and effective tool for studying Aichi virus infection mechanisms and potential interventions.
Provides a novel pseudovirus tool for studying Aichi virus pathogenesis; leaves open clinical applications pending.
Human Aichi virus (AiV) (genus Kobuvirus, family Picornaviridae) has been described as a causative agent of human gastroenteritis since 1989. However, research on AiV at cellular and animal levels is limited. Utilizing a double reporter gene system, we constructed an AiV capsid protein plasmid and genomic backbone Replicon. Subsequently, AiV pseudovirus (AiV PsV) particles were packaged using a three-plasmid co-transfection system. Eleven cell types were screened to identify those susceptible to AiV PsV. Mouse models were established with AiV PsV to determine the optimal mouse species, mode of infection, and detection time, and investigate distribution characteristics of AiVs in vivo. HeLa cells exhibited the highest sensitivity to AiV PsV. A BALB/c mouse model established with bioluminescence imaging performed 24 h after infection via intraperitoneal injection demonstrated that the bioluminescent signal was concentrated in the murine abdominal cavity. The AiV PsV system should advance understanding of the infectious features of AiVs.
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Wu et al. (2025) studied Human Aichi virus infection. AiV pseudovirus system was evaluated. A novel pseudovirus system for human Aichi virus was successfully developed, demonstrating high susceptibility in HeLa cells and enabling a bioluminescent imaging infection model in BALB/c mice.
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