Key result
A rapid vacuum-driven procedure using Sep-Pak C18 cartridges achieved mean recoveries of 96.4% for AVP and 94.8% for ANP, validating its use in clinical plasma samples.
A rapid vacuum-driven procedure using Sep-Pak C18 cartridges allows for the simultaneous and reliable extraction of AVP and ANP from plasma for radioimmunoassay.
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May enable simultaneous AVP/ANP extraction in clinical labs; leaves open independent validation before routine use.
A rapid vacuum-driven procedure, using pre-treated Sep-Pak C18 cartridges, has been developed for the simultaneous extraction of arginine vasopressin (AVP) and atrial natriuretic peptide (ANP) from plasma. Non-specific interference was removed by fractional elution with an aqueous methanol/trifluoroacetic acid (TFA) mixture. AVP and ANP were coeluted under positive pressure with a methanol/TFA mixture and the eluates air-dried before measurement using separate radioimmunoassays. Assay ranges for AVP and ANP were 0.12-29.5 pmol/L and 0.65-162 pmol/L, respectively, with mean recoveries (standard deviation in parentheses) for AVP of 96.4% (5.5%) at a level of 11.8 pmol/L and for ANP of 94.8% (5.9%) at a level of 32.4 pmol/L. The extraction and assay procedures were validated by observing the changes in plasma AVP and ANP concentrations in normal subjects at different stages of hydration and in elderly patients during treatment for congestive cardiac failure.
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Penney et al. (1992) studied Congestive cardiac failure. Vacuum-driven procedure using Sep-Pak C18 cartridges for plasma extraction was evaluated on Assay ranges and mean recoveries for AVP and ANP. A rapid vacuum-driven procedure using Sep-Pak C18 cartridges achieved mean recoveries of 96.4% for AVP and 94.8% for ANP, validating its use in clinical plasma samples.
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