What question did this study set out to answer?

To develop and validate a semi-automated, macro-based digital image analysis method for quantifying myocardial fibrosis compared against conventional microscopy and stereology.

May 2, 2011Cellular OncologyOpen Access

Rapid quantification of myocardial fibrosis: a new macro-based automated analysis

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Population

Adult Wistar rats with monocrotaline-induced pulmonary hypertension and controls, and paraffin-embedded…

Comparison

Semi-automated quantification technique for… vs Traditional polarisation microscopy and…

Design

Preclinical

Authors

KMKoen T. B. MouchaersAmsterdam UMC Location Vrije Universiteit Amsterdam ISIngrid SchalijAmsterdam University Medical Centers KGKatrien GrünbergLeiden University

Discussion

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Implication

May expedite preclinical fibrosis quantification; leaves open clinical translation pending prospective human validation.

Key Points

To develop and validate a semi-automated, macro-based digital image analysis method for quantifying myocardial fibrosis compared against conventional microscopy and stereology.
Induced pulmonary hypertension (PH) in adult Wistar rats via monocrotaline injection (40 mg/kg; n=9 PH vs. n=9 control) and stained 5 μm myocardial cryosections with Picrosirius red.
Developed an automated ImageJ macro to quantify fibrosis across 21 digital slide images per heart, comparing results to polarization microscopy and gold-standard stereology.
Evaluated the automated macro across paraffin-embedded human heart, lung, kidney, and liver tissue specimens.
Automated analysis identified significant increases in myocardial fibrosis in PH hearts compared to controls, correlating strongly with polarization microscopy (r² = 0.92) and stereology (r² = 0.95).
Human tissue analyses across heart, lung, kidney, and liver demonstrated high correlation with stereology (r² = 0.91).
Automated analysis required only 22% of the processing time needed for stereology and 33% of the time required for polarization microscopy.

Structured PICO

Population

Adult Wistar rats with monocrotaline-induced pulmonary hypertension (n=9) and controls (n=9), and paraffin-embedded human tissues (heart, lungs, kidney, liver).

Intervention

Semi-automated quantification technique for myocardial fibrosis using an ImageJ macro.

Comparator

Traditional polarisation microscopy and stereology.

Outcome

Correlation of automated analysis with traditional polarisation and stereology analysis, and time required for analysis.surrogate

A new semi-automated ImageJ macro for quantifying myocardial fibrosis is highly correlated with traditional methods but significantly faster.

Cite This Study

Mouchaers et al. (2011) studied this question.

synapsesocial.com/papers/6a8e8936864bbf5089e259a6 https://doi.org/10.1007/s13402-011-0035-7

Topics

Cardiomyopathy

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Rapid quantification of myocardial fibrosis: a new macro-based automated analysis

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Topics

Rapid quantification of myocardial fibrosis: a new macro-based automated analysis

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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