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June 1, 1980Proceedings of the National Academy of SciencesOpen Access

Identification of an N2 line protein of striated muscle.

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Population

Rabbit psoas myofibrils

Design

Preclinical

Authors

KWKuan WangSecond Military Medical UniversityCWC L Williamson

Discussion

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Implication

May inform sarcomere protein mapping in skeletal muscle; leaves open relevance to human cardiac titinopathies.

Key Points

  • To purify and characterize the large myofibrillar protein band 3 and determine its structural localization within skeletal muscle sarcomeres.
  • Purified the high-molecular-weight protein band 3 (molecular weight 500,000–600,000) from rabbit psoas myofibrils.
  • Conducted chemical and immunochemical differentiation against titin and myosin heavy chain, followed by immunofluorescence localization in glycerinated myofibrils across sarcomere lengths of 1.5–3.7 µm.
  • Band 3 was confirmed to be chemically and immunochemically distinct from titin and myosin heavy chain.
  • In sarcomeres measuring 1.8–3.7 µm, band 3 localized predominantly to the N2 line in the I band, maintaining a proportional distance from the Z and M lines during elongation.
  • In contracted sarcomeres measuring 1.5–1.8 µm, band 3 localized primarily within the A band, demonstrating a biphasic distribution.

Structured PICO

P
Population
Rabbit psoas myofibrils
I
Intervention
Purification and immunofluorescent localization of "band 3" protein
O
Outcome
Localization of band 3 protein in sarcomeressurrogate

Identifies "band 3" as an N2 line component of rabbit skeletal myofibrils, distinct from titin and myosin heavy chain.

Cite This Study

Wang et al. (1980) studied this question.

synapsesocial.com/papers/6a8e9e1496767b15a7fff4bfhttps://doi.org/10.1073/pnas.77.6.3254
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Titin: major myofibrillar components of striated muscle.1979 · 548 citations
  2. 2Compositional studies of myofibrils from rabbit striated muscle.1976 · 163 citations
  3. 3Cytoplasmic microtubular images in glutaraldehyde-fixed tissue culture cells by electron microscopy and by immunofluorescence microscopy1978 · 257 citations
  4. 4Muscle Filament Structure and Muscle Contraction1975 · 126 citations
  5. 5Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.1979 · 53,817 citations